王晓峰

发布日期:2017-09-15      浏览次数:     作者:     来源:

 1.基本信息

王晓峰,男,陕西咸阳人,西北农林科技大学旱区作物逆境生物学国家重点实验室研究人员,园艺学院教授、博士生导师。在西北农业大学园艺系学习,先后获学士和硕士学位,19937月留校任教。1999年在西北农业大学获博士学位。2000年至2002年在以色列农业研究中心(Volcani Center)和以色列魏兹曼研究所(Weizmann Institute)做博士后研究,2002年至2010年在美国北卡罗来纳州州立大学(NC State University)从事科研工作。

现为陕西省百人计划特聘教授,陕西省政府特聘专家,陕西省园艺学会理事,美国高级科学研究学会Sigma XI及美国植物学家学会会员

 2.研究方向和研究兴趣

主要从事植物生化和分子生物学的研究及其在园艺作物代谢调控分子育种上的应用, 内容包括植物组织培养及遗传转化,基因克隆和功能分析,植物生长发育过程中的基因表达调控,植物激素信号传导的分子机制,植物信号传导的功能基因组学和蛋白组学等。研究兴趣主要为油菜素受体(BRI1) 及其相关受体(BAK1) 的互作组分及信号传导的分子机理, 膜蛋白受体的生物学功能和相互作用等,并致力于将这些基础研究的成果应用于园艺作物的栽培和育种实践。

应用免疫沉淀及液相色谱与质谱连用(LC-MS/MS),对BRI1(油菜素受体) 和BAK1(油菜素受体相关激酶受体)的活体磷酸化位点进行了系统的鉴定,首次在植物界建了完整的受体激酶活体磷酸化图谱。借助单个位点的定向突变对所鉴定的磷酸化位点的生化和生物学功能进行了评估。通过对BRI1和BAK1复合体形成和磷酸化的研究, 以及对BRI1 和BAK1 的信号分解,揭示了油菜素信号传导分子机理的几个重要方面,建立了油菜素早期信号传导模型。近年来在番茄、白菜和马铃薯等蔬菜作物上,通过磷酸化位点突变的基因的过表达和基因敲除编辑,研究油菜素内酯路径组分的磷酸化位点的农艺学价值及其在分子育种上的应用,以期通过人为操控磷酸化位点的磷酸化,精准调控油菜素内酯信号的强度和特异性,进而改良作物的重要农艺性状。

  3. 开设课程

主讲课程包括《园艺植物生物技术》、《蔬菜栽培学》、《食用菌栽培学》、《新生研讨课》、《科研基本方法》、《瓜果类蔬菜栽培学》、《蔬菜学研究进展》等。

  4. 主要研究论文

1) Imkampe J, Halter T, Huang S, Schulze S, Mazzotta S, Schmidt N, Manstretta R, Postel S, Wierzba M, Yang Y2, vanDongen WM, Stahl M, Zipfel C, Goshe MB, Clouse S, de Vries SC, Tax F, Wang X*, Kemmerling B* .(2017) The Arabidopsis Leucine-rich Repeat Receptor Kinase BIR3 Negatively Regulates BAK1 Receptor Complex Formation and Stabilizes BAK1. Plant Cell. Aug 25. pii: tpc.00376.2017. doi: 10.1105/tpc.17.00376. [Epub ahead of print]

2) Shuhua Huang , Shuming Nie , Shufen Wang , Jianwei Liu , Yanfeng Zhang , Xiaofeng Wang*. SlBIR3 (2017) Negatively Regulates PAMP Responses and Cell Death in Tomato. International Journal of Molecular Sciences. 2017 Sep 13;18(9). pii: E1966. doi: 10.3390/ijms18091966.

3) Shuming Nie , Shuhua Huang , Shufen Wang , Dandan Cheng , Jianwei Liu , Siqi Lv , Qi Li , Xiaofeng Wang *. Enhancing Brassinosteroid Signaling via Overexpression of Tomato (Solanum lycopersicum) SlBRI1 Improves Major Agronomic Traits. Front Plant Sci. 2017 Aug 10;8:1386. doi: 10.3389/fpls.2017.01386. eCollection 2017.

4) Cheng Li, Shan Zhang, Xiaofeng Wang*. (2017) Novel Signaling Interface Constituted with Membrane Receptor-Like Kinases Emerged from the Study of Interaction and Transphosphorylation of BRI1 and BAK1. Current Topics in Medicinal Chemistry17(21):2393-2400. doi: 10.2174/1568026617666170414144145

5) Qiannan Wang, Shufen Wang, Sufu Gan, Xin Wang, Jianwei Liu, Xiaofeng Wang*. (2016) Role of Specific Phosphorylation Sites of Arabidopsis Brassinosteroid-Insensitive 1 Receptor Kinase in Plant Growth and Development. Journal of Plant Growth Regulation. 35(3): 755-769 .  DOI 10.1007/s00344-016-9580-7

6) Xin Wang, Dongli Gao, Jinjing Sun, Min Liu, YaoYao Lun, Jianshu Zheng, Shenhao Wang, Qingzhi Cui, Xiaofeng Wang*, Sanwen Huang*. (2016) An exon skipping in a SEPALLATA-Like gene is associated with perturbed floral and fruits development in cucumber. Journal of Integrative Plant Biology. doi: 10.1111/jipb.12472.

7) Shan Zhang, Cheng Li, Qi Li, Qian Nan Wang, Shu Hua Huang, Yan Feng Zhang and Xiao Feng Wang*. (2015)  Functional divergence of BAK1 Genes from Brassica rapa in regulating plant architecture. Genetics and Molecular Research. 14 (4): 14587-14596

8) Mitra SK, Chen R, Dhandayham M, Wang xiaofeng, Blackburn RK, Kota U,Goshe MB, Schwarts D, Huber SC, Clouse SD*. (2015) An autophosphorylation site database for leucine-rich repeat receptor-like kinases in Arabidopsis thaliana. Plant Journal:82(6):1042-60.

9) Vikramjit S. Bajwa, Xiaofeng Wang, R. Kevin Blackburn, Michael B. Goshe, Srijeet K. Mitra, Elisabeth L. Williams, Gerard J. Bishop, Sergei Krasnyanski, George Allen, Steven C. Huber, and Steven D. Clouse*. (2013) Identification and Functional Analysis of Tomato BRI1 and BAK1 Receptor Kinase Phosphorylation Sites. Plant Physiology: 163 (1): 30-42.

10) Man-Ho Oh, Xiaofeng Wang, Steven D. Clouse, and Steven C. Huber*.2012 Deactivation of the Arabidopsis BRI1 receptor kinase by autophosphorylation within the glycine-rich loop involved in ATP binding. Proc Natl Acad Sci U S A, 109(1):327-332.

11) Man-Ho Oh, Xiaofeng Wang, Uma Kota, Michael B. Goshe, Steven D. Clouse, and Steven C. Huber*. (2009) Tyrosine phosphorylation of the BRI1 receptor kinase emerges as a component of Brassinosteroid signaling in Arabidopsis. Proc Natl Acad Sci U S A, 106 (2):658-663

12) Xiaofeng Wang, Uma Kota, Kai He, Kevin Blackburn, Jia Li, Michael B. Goshe, Steven C. Huber, Steven D. Clouse*. (2008) Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in Brassinosteroid signaling. Developmental Cell, 15(2):220-235

13) Xiaofeng Wang, Michael B. Goshe, Erik J. Soderblom, Brett S. Phinney, Jason A. Kuchar, Jia Li, Tadao Asami, Shigeo Yoshida, Steven C. Huber, Steven D. Clouse*. (2005) Identification and Functional Analysis of in vivo Phosphorylation Sites of the Arabidopsis BRASSINOSTEROID INSENSITIVE 1 Receptor Kinase. The Plant Cell, 17: 1685-1170

14) Lian-Hai Fu, Xiao-Feng Wang, Yoram Eyal, Gozal Ben-Hayyim*, et al.(2002) A selenoprotein in the plant kingdom: mass spectrometry confirms that an opal codon (UGA) encodes selenocysteine in Chamydomonas reinhadtii glutathion peroxidase. J. Biol. Chem., 277(29):25983-25991

  5. 联系方式

  通讯地址:陕西杨凌邰城路3号 西北农林科技大学园艺学院

  邮编:712100

Email: wangxff99@nwsuaf.edu.cn; wangxf9993@yahoo.com